os Projeto Angiogênese: Abordagem Biomédica E Estratégia De Extração Da Fração Frhbi Do Látex Natural Comercial
O Projeto Angiogênese: Abordagem Biomédica E Estratégia De Extração Da Fração Frhbi Do Látex Natural Comercial
Abstract
Tissue engineering is a multidisciplinary field that aims to develop biomaterials capable of repairing or replacing damaged tissues and organs. Natural Rubber Latex (NRL) has been extensively studied due to its biocompatible properties, flexibility, and structural similarity to the extracellular matrix. The FrHB1 protein fraction, present in NRL, has shown potential in tissue regeneration and wound healing. However, most studies focus on extracting this fraction directly from latex collected from the rubber tree, without considering the use of commercial natural latex, which could offer significant advantages for translational research and the development of scalable biomaterials.
This study presents an innovative methodology for the extraction and purification of the FrHB1 fraction from Dulatex commercial natural latex, marking the first documented approach for this type of material. Protein quantification was performed using the Bradford assay, yielding an average final concentration of 0.196 ± 0.030 mg/mL for FrHB1. Purification was carried out using liquid chromatography with the ÄKTA system, employing NaCl gradients of 0.25 mol/L, 0.5 mol/L, and 1.5 mol/L for protein fraction separation. The chromatographic profile demonstrated the method’s efficiency, ensuring the isolation of a purified fraction suitable for biomedical studies.
The use of commercial latex represents a significant step forward in translational research, as it enables greater reproducibility of experiments, reduces variability in raw materials, and facilitates the clinical application of results. Moreover, the FrHB1 fraction has shown potential for use in tissue engineering scaffolds, controlled drug delivery systems, and biocompatible coatings for medical devices. Further studies will be necessary to assess its immunological response and integration with different biomaterials.
In conclusion, the developed methodology can contribute to the production of innovative biomaterials and promote new regenerative therapies, reinforcing NRL as a promising biomedical resource.
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